Transcriptomic analyses provide insight into adventitious root formation of Euryodendron excelsum H. T. Chang during ex vitro rooting

Euryodendron excelsum H. T. Chang, a critically endangered species endemic to China, is source of valuable material for the furniture and construction industries. However, this has some challenges associated with rooting during in vitro propagation that have yet be resolved. In study, we optimized conducted transcriptomic analysis appreciate its molecular mechanism, thereby promoting practical application E. excelsum, providing technical support ecological protection rare species. Results showed ex performed highest percentage 98.33% at 25 days. During rooting, there was wide fluctuation endogenous levels indole-3-acetic acid (IAA) hydrogen peroxide (H2O2) stage root primordia formation. Transcriptome revealed multiple differentially expressed genes (DEGs) involved adventitious (AR) development. DEGs plant hormone signal transduction, such as encoding auxin-induced protein, auxin-responsive auxin transporter-like response H2O2, oxidative stress, abiotic biotic stimuli were significantly up- or down-regulated by treatment 1 mM indole-3-butyric (IBA). Our results indicate an effective method induce AR from plantlets micropropagation. transduction pathway played crucial role produced environmental stimulation, might related induction result synergistic action IBA, ultimately regulating level IAA. Under between H2O2 stimulation IBA regulation formation